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Evident Corporation hoffman modulation contrast microscope
Hoffman Modulation Contrast Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoffman+modulation+contrast+microscope/inverted+light+microscope/pmc11285943-259-10-11
Average 90 stars, based on 1 article reviews
hoffman modulation contrast microscope - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: PITHD1 is a proteasome-interacting protein essential for male fertilization
Article Snippet: .. The eggs were incubated with 2.0 × 10 5 sperm for 6 h at 37 °C, washed with TYH medium, further incubated for 18 h, and observed under a Hoffman modulation contrast microscope (IX71; Olympus). ..

Article Title: PITHD1 is a proteasome-interacting protein essential for male fertilization
Article Snippet: .. The eggs were incubated with 2.0 × 105 sperm for 6 h at 37 °C, washed with TYH medium, further incubated for 18 h, and observed under a Hoffman modulation contrast microscope (IX71; Olympus). ..

Article Title: A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility
Article Snippet: .. The eggs were incubated with 2.0 × 10 5 sperm for 6 h at 37°C, washed with TYH medium, further incubated for 18 h, and observed using a Hoffman modulation contrast microscope (IX71; Olympus). .. To assay the ability of sperm to bind the zona pellucida layer, sperm (2.5 × 10 5 ) were incubated at 37°C for 30 min with 10 cumulus-free eggs in 100 μl of TYH medium, fixed with 0.1% PFA, and observed by the use of an Olympus IX-71 microscope.

Article Title: A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility
Article Snippet: .. The eggs were incubated with 2.0 105 sperm for 6 h at 37°C, washed with TYH medium, further incubated for 18 h, and observed using a Hoffman modulation contrast microscope (IX71; Olympus). .. To assay the ability of sperm to bind the zona pellucida layer, sperm (2.5 105) were incubated at 37°C for 30 min with 10 cumulus-free eggs in 100 l of TYH medium, fixed with 0.1% PFA, and observed by the use of an Olympus IX-71 microscope.

Microscopy:

Article Title: PITHD1 is a proteasome-interacting protein essential for male fertilization
Article Snippet: .. The eggs were incubated with 2.0 × 10 5 sperm for 6 h at 37 °C, washed with TYH medium, further incubated for 18 h, and observed under a Hoffman modulation contrast microscope (IX71; Olympus). ..

Article Title: Establishment of Intracytoplasmic Sperm Injection Technique in Japanese Quail and its Possible Application for Poultry Resources and Transgenic Birds
Article Snippet: Microinjection procedures were performed under a stereomicroscope using a micromanipulator (ONM-1; Narishige) connected to the injector and an image processor system. .. A single sperm was picked up tail first into an injection pipette (Fig. 2) under a Hoffman modulation contrast microscope (IX70, Olympus). .. The ovum was placed into Dulbecco’s modified Eagle's medium (DMEM) in the plastic dish (six-well plastic dish), and the sperm was injected into the injector (PMM-MB-U; Prime Tech) with silicon tubing filled with silicon oil, under a stereomicroscope (SZ11, Olympus) while being monitored (SSM-121, Sony) with the aid of an image-processor system (Image ΣIII, Nippon Avionics).

Article Title: Downregulation of the microtubule associated protein tau impairs process outgrowth and myelin basic protein mRNA transport in oligodendrocytes.
Article Snippet: Oligodendrocytes, the myelin forming cells of the CNS, are characterized by their numerous membranous extensions, which enwrap neuronal axons and form myelin sheaths.. During differentiation oligodendrocytes pass different morphological stages, downregulate the expression of the proteoglycan NG2, and acquire major myelin specific proteins, such as myelin basic proteins (MBP) and proteolipid protein.. MBP mRNA is transported in RNA granules along the microtubules (MTs) to the periphery and translated locally.

Article Title: PITHD1 is a proteasome-interacting protein essential for male fertilization
Article Snippet: .. The eggs were incubated with 2.0 × 105 sperm for 6 h at 37 °C, washed with TYH medium, further incubated for 18 h, and observed under a Hoffman modulation contrast microscope (IX71; Olympus). ..

Article Title: A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility
Article Snippet: .. The eggs were incubated with 2.0 × 10 5 sperm for 6 h at 37°C, washed with TYH medium, further incubated for 18 h, and observed using a Hoffman modulation contrast microscope (IX71; Olympus). .. To assay the ability of sperm to bind the zona pellucida layer, sperm (2.5 × 10 5 ) were incubated at 37°C for 30 min with 10 cumulus-free eggs in 100 μl of TYH medium, fixed with 0.1% PFA, and observed by the use of an Olympus IX-71 microscope.

Article Title: Spermatogenic cell-specific type 1 hexokinase (HK1S) is essential for capacitation-associated increase in tyrosine phosphorylation and male fertility in mice
Article Snippet: .. Real-time videos were recorded immediately after isolation, at 10 min and 2 h after isolation using Hoffman Modulation Contrast microscope (OLYMPUS IX71). .. A CASA system (Version.14 CEROS, Hamilton Thorne Research, USA) with a Slide Warmer (#720230, Hamilton Thorne Research, USA) was used to quantify parameters of sperm motility with the following settings: minimal contrast, 30; minimal cell size, 4 pixels; and 30 frames were acquired at a frame rate of 60 Hz.

Article Title: Spermatogenic cell-specific type 1 hexokinase (HK1S) is essential for capacitation-associated increase in tyrosine phosphorylation and male fertility in mice
Article Snippet: .. Two-cells were counted the next day using Hoffman Modulation Contrast microscope (OLYMPUS IX71). ..

Article Title: A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility
Article Snippet: .. The eggs were incubated with 2.0 105 sperm for 6 h at 37°C, washed with TYH medium, further incubated for 18 h, and observed using a Hoffman modulation contrast microscope (IX71; Olympus). .. To assay the ability of sperm to bind the zona pellucida layer, sperm (2.5 105) were incubated at 37°C for 30 min with 10 cumulus-free eggs in 100 l of TYH medium, fixed with 0.1% PFA, and observed by the use of an Olympus IX-71 microscope.

Injection:

Article Title: Establishment of Intracytoplasmic Sperm Injection Technique in Japanese Quail and its Possible Application for Poultry Resources and Transgenic Birds
Article Snippet: Microinjection procedures were performed under a stereomicroscope using a micromanipulator (ONM-1; Narishige) connected to the injector and an image processor system. .. A single sperm was picked up tail first into an injection pipette (Fig. 2) under a Hoffman modulation contrast microscope (IX70, Olympus). .. The ovum was placed into Dulbecco’s modified Eagle's medium (DMEM) in the plastic dish (six-well plastic dish), and the sperm was injected into the injector (PMM-MB-U; Prime Tech) with silicon tubing filled with silicon oil, under a stereomicroscope (SZ11, Olympus) while being monitored (SSM-121, Sony) with the aid of an image-processor system (Image ΣIII, Nippon Avionics).

Transferring:

Article Title: Establishment of Intracytoplasmic Sperm Injection Technique in Japanese Quail and its Possible Application for Poultry Resources and Transgenic Birds
Article Snippet: Microinjection procedures were performed under a stereomicroscope using a micromanipulator (ONM-1; Narishige) connected to the injector and an image processor system. .. A single sperm was picked up tail first into an injection pipette (Fig. 2) under a Hoffman modulation contrast microscope (IX70, Olympus). .. The ovum was placed into Dulbecco’s modified Eagle's medium (DMEM) in the plastic dish (six-well plastic dish), and the sperm was injected into the injector (PMM-MB-U; Prime Tech) with silicon tubing filled with silicon oil, under a stereomicroscope (SZ11, Olympus) while being monitored (SSM-121, Sony) with the aid of an image-processor system (Image ΣIII, Nippon Avionics).

Fluorescence:

Article Title: Downregulation of the microtubule associated protein tau impairs process outgrowth and myelin basic protein mRNA transport in oligodendrocytes.
Article Snippet: Oligodendrocytes, the myelin forming cells of the CNS, are characterized by their numerous membranous extensions, which enwrap neuronal axons and form myelin sheaths.. During differentiation oligodendrocytes pass different morphological stages, downregulate the expression of the proteoglycan NG2, and acquire major myelin specific proteins, such as myelin basic proteins (MBP) and proteolipid protein.. MBP mRNA is transported in RNA granules along the microtubules (MTs) to the periphery and translated locally.

Isolation:

Article Title: Spermatogenic cell-specific type 1 hexokinase (HK1S) is essential for capacitation-associated increase in tyrosine phosphorylation and male fertility in mice
Article Snippet: .. Real-time videos were recorded immediately after isolation, at 10 min and 2 h after isolation using Hoffman Modulation Contrast microscope (OLYMPUS IX71). .. A CASA system (Version.14 CEROS, Hamilton Thorne Research, USA) with a Slide Warmer (#720230, Hamilton Thorne Research, USA) was used to quantify parameters of sperm motility with the following settings: minimal contrast, 30; minimal cell size, 4 pixels; and 30 frames were acquired at a frame rate of 60 Hz.



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Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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Image Search Results


Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Journal: Cells

Article Title: The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells

doi: 10.3390/cells10102723

Figure Lengend Snippet: Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Tumorspheres were grown for 4 days at 37 °C/5% CO 2 and visualized in an Olympus IX70 microscope equipped with Phase Contrast and Hoffman Modulation Contrast, and a SPOT imaging system.

Techniques: Western Blot, Transfection, Microscopy, Software